#Compensation flowjo full

This will force a recalculation of all the contents of this window. If you see a gate that needs adjusting, double-click the plot to open it in a graph window, and adjust the gate.Lastly on the bottom, you will find plots for the gates FlowJo is using to compute the matrix: To remove extra parameters or add additional parameters the compensation editor may have missed, click on the sample drop down menu next to any samples and choose the appropriate option. Speaker: Veronica Obregon-Perko Date: OctoTime: 10:00 AM PDT Join. You can make your own gates in the workspace and also use those by selecting them in the drop down menu under negative and positive assignments. Supervised Autogating Tools in FlowJo v10.

#Compensation flowjo full

All of the clickable buttons (Sample, Negative, Positive) can be used to change the current selection, and will force a full recalculation of the Compensation window.

The Positive column shows which gate FlowJo is currently using as a “positive” control gate.

The Negative column shows which gate FlowJo is currently using as a “negative” control gate.

From the dropdown menu in the Negative column the user has the option to.

The Sample column links a specific file from the controls to the specific color (parameter) from the first list. The compensation wizard in FlowJo will auto-gate what will typically be a monolithic population on the clean-up gate of unstained cells, deem it the ‘positive’ population, and calculate the median fluorescent intensity (MFI) of this population in the selected channel.

The left-most column is a list of parameters FlowJo found in the samples present in the current Compensation Group. From FlowJo’s Daily Dongle Three Rules for Compensation Controls First and foremost, there must be a single-stained control for every parameter in the experiment In addition, there are three.

The next section is a matrix of parameters vs samples vs control gates, and is where you can examine/assign the roles of your different controls:

Matrix Editor button will open a new window with more plots and detailed info about the current matrix – for help with that interface, click here.

The “apply” pull-down will send the matrix resulting from this window into the samples you select in the pulldown – ie samples in current group, or samples without compensation, etc.

The compensation matrix is represented by the grid to the left of the sample name.Find more FlowJo tuto.

The drag contains the current compensation matrix – it can be dropped on samples/groups in FJ to compensate files quickly or it can be dropped on the operating system file system to export the matrix. Create new, apply old, or compare different matrices. The leftmost button’s purpose is to be clicked and dragged.

The Compensation Node will be named/styled by these settings: The top part of the window deals with matrix settings. This is a complex window, let’s examine it one part at a time. This will open the main Compensation Window: To open this window, click on your Compensation group to select it, then click the tool menu tab and select Compensation: This page describes the compensation window.